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>> > > Hi, Mr. Lang. It'' s Dr. Hayes, the epidemiologist from the state health department. >> > > Hi, Dr. Hayes. >> > > I hope you'' re sensation much better because our last telephone call regarding your Salmonella infection. I'' m calling because others are ill with the very same microorganisms as well as we'' re worried that this could be a break out. Can I ask you some more concerns regarding what you consumed lately? >> > > Sure. I'' m delighted to answer your questions. I'' d hate for any individual else to obtain ill. Just how do you recognize we have the very same germs? >> > > Well, the lab determined that the DNA fingerprint of the germs that made you ill was really comparable to the finger prints of microorganisms that have actually made various other individuals unwell around the nation. Among our laboratory researchers, Dr. Gray, is right here with me. She can describe how the lab recognized the microorganisms. >> > > Nice to talk with you, Mr. Lang. Are you really feeling far better? >> > > A great deal far better, thanks.I never wish to
obtain that ill once more.
How did you identify I have the same Salmonella that
' s making others sick? Dr. Hayes discussed some type of DNA finger print.
> > Yes, DNA, more specifically>, the DNA series, is what we utilize to establish if people are unwell with the same kind of germs.
It ' s a complicated process that makes use of a stool example.
Right here ' s just how it functions. When'we received your feces sample, we streaked it on a culture plate that allows all the bacteria in your feces to
expand, including the germs that made you ill.
The nests from each bacteria can look various on a plate and also we can recognize them based on their look along with other lab tests.In your example, we recognized Salmonella.

All microorganisms have genomes comprised of DNA. The DNA series informs the microorganisms how to consume, grow, and also multiply.
It ' s distinct to every microorganisms and can be referred to as a “DNA finger print.” For episode examinations, we contrast the DNA fingerprint of the germs that made somebody ill to the fingerprint from an additional ill patient.
If the two DNA fingerprints are similar or extremely comparable, then we have a suit. > > So exactly how do you read a bacteria ' s genome to recognize what its DNA fingerprint looks
like? > > Excellent concern. The process is called “whole genome sequencing.” Every >> microbial cell has a genome”that has DNA.” We take countless genomes as well as reduce them right into pieces little enough to fit right into a DNA sequencing device. > > Millions? > > Yes.Then we mix them
with each other and also feed the items right into the >> device.

The details is sent out to a computer as well as reconstructed into a single duplicate of the genome, so we can contrast one bacterial genome to others.
> > That seems like a. truly complicated puzzle. > > Yes, it is. >> That ' s why we have. actually effective computers. >> We then compare the'DNA.
finger print of the germs that made you unwell to.
those of the bacteria that made others sick.
> > Which ' s where. we come back in. >> When the lab tells.
us that numerous of the exact same bacterial DNA. finger prints have actually been determined, we start. investigating to figure out if we have an
break out. as well as, if so, what ' s creating it. We ask people, like you,. concerning the foods they ate and the things they carried out in.
the weeks before they obtained ill to attempt and also find an usual.
food among every person. >> > > Wow, that'' s great. > > Yes, it is. We have a network called.
PulseNet that enables us to connect foodborne.
illnesses throughout the country to.
identify break outs. This network has actually avoided.
270,000 people from getting ill and saved the U.S.Over fifty percent

a billion dollars every year. Whole genome sequencing has.
made PulseNet even much better. >> > > That ' s amazing. >> > > PulseNet works due to the fact that.
of people like you. You aided us by mosting likely to.
your physician when you got sick as well as answering our concerns.
concerning where you were, what you were doing, and also what.
you ate prior to you obtained unwell. Collaborating, we can determine infected.
food quickly before more individuals obtain sick. That'' s ultimately our goal.

>> > > Many thanks for explaining.
whole genome sequencing. Dr. Hayes, I'' m prepared to.
address your concerns.

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